Major Outer Membrane Proteins: Common Antigens in Enterobacteriaceae Species
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چکیده
منابع مشابه
ISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99
Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...
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Outer membrane extracts of Treponema hyodysenteriae were used to evaluate the antibody responses in immunized or convalescent pigs. Western blot (immunoblot) analysis identified antibodies in sera reactive with 14- to 19-kilodalton (kDa) antigens. Reactivity against these antigens could be removed only by absorption of sera with butanol-water-extracted endotoxin from the homologous strain of T....
متن کاملCharacteristics of major outer membrane proteins of Haemophilus influenzae.
Several properties of Haemophilus influenzae outer membrane proteins were analyzed to define related proteins in various isolates. H. influenzae type b 760705 had six major outer membrane proteins with the following characteristics. Protein a (Mr, 47,000) demonstrated heat modifiability in sodium dodecyl sulfate; its apparent molecular weight was 34,000 at temperatures below 60 degrees C. This ...
متن کاملisolation and purification of major outer membrane proteins from brucella abortus s-99
isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...
متن کاملPredominant outer membrane antigens of Bartonella henselae.
A hallmark of Bartonella henselae is persistent bacteremia in cats despite the presence of a vigorous host immune response. To understand better the long-term survival of B. henselae in cats, we examined the feline humoral immune response to B. henselae outer membrane (OM) proteins in naturally and experimentally infected cats. Initially, a panel of sera (n = 42) collected throughout North Amer...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1980
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-119-1-123